Device

Part:BBa_K3702205:Design

Designed by: Dat Vo, Christine Cremeans   Group: iGEM20_FSU   (2020-10-10)


Modified EreA+EreB generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 397
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1475
    Illegal XhoI site found at 1885
    Illegal XhoI site found at 2475
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1602


Design Notes

This device was designed to generate EreA and EreB in an E. Coli cell to degrade macrolide antibiotics. Since EreB is a more well documented enzyme, it was a more reliable enzyme to depend on than EreA, so a stronger RBS was used for ereB. The two specific RBS were chosen because the strength test for these RBS has been done in the past. We couldn't test different RBS since we did not have a lab this year, but relative strength of the two RBS could be found here (https://parts.igem.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Community_Collection).

Source

https://parts.igem.org/Part:BBa_J23100 https://parts.igem.org/Part:BBa_B0030 https://parts.igem.org/Part:BBa_K3702177 https://parts.igem.org/Part:BBa_B0034 https://parts.igem.org/Part:BBa_K1159000 https://parts.igem.org/Part:BBa_B0015

References